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coomassie brilliant blue staining solution  (Bio-Rad)


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    Bio-Rad coomassie brilliant blue staining solution
    Coomassie Brilliant Blue Staining Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2212 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coomassie+blue+solution/Coomassie+Brilliant+Blue+R-250+Staining+Solution/pm42122151-87-19-32
    Average 99 stars, based on 2212 article reviews
    coomassie brilliant blue staining solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Enhancing immunogenicity of
    Article Snippet: The plate was vacuum filtered and 100 μl of Todd Hewett yeast extract (THYE, Teknova) broth was added. .. The medium was filtered and the moist plate was placed in a sealed plastic bag overnight at 27° C. Plate filters were then stained with 100 μl/well of a 0.1% Coomassie blue solution (Bio-Rad, Hercules, CA). .. Stain was filtered through the plate, colonies were destained with Coomassie destaining solution (Bio-Rad) and vacuum filtered again until dry.

    Article Title: Hortaea werneckii.
    Article Snippet: The initiation of this study relies on a targeted genome-mining approach to highlight the presence of a putative vanadiumdependent haloperoxidase-encoding gene in the deep-sea hydrothermal vent fungus Hortaea werneckii UBOCC-A-208029.. To date, only three fungal vanadium-dependent haloperoxidases have been described, one from the terrestrial species Curvularia inaequalis, one from the fungal plant pathogen Botrytis cinerea, and one from a marine derived isolate identified as Alternaria didymospora.. In this study, we describe a new vanadium chloroperoxidase from the black yeast H. werneckii, successfully cloned and overexpressed in a bacterial host, which possesses higher affinity for bromide (Km = 26 μM) than chloride (Km = 237 mM).

    Article Title: Storage protein biosynthesis is affected by ionome composition in soybean ( Glycine max (L.) Merrill) seeds
    Article Snippet: .. After that, the gels were carefully removed from the cassette and stained with 0.25% Coomassie Blue solution (0.25% Coomassie Brilliant Blue R250, 90% methanol: H 2 O (1:1 v/v), 10% glacial acetic acid) for 30-min, then destained overnight (60% H 2 O, 30% methanol, 10% acetic acid), and imaged at a ChemiDoc MP Imaging System (Bio-Rad, USA). ..

    Article Title: Concanavalin A-functionalized nanogold – a glycan tag for surface enhanced Raman spectroscopy
    Article Snippet: Electrophoresis was carried out in Tris–HCl-Glycine-SDS running buffer (pH 8.3) at 150 V for 45 min (Mini-PROTEAN®, BioRad US), using a molecular marker (ColorBurstTM 8000–220,000 Da, Sigma-Aldrich). .. Following electrophoresis, the gel was stained using a Coomassie blue solution (Coomassie Brilliant Blue R-250, BioRad, US). .. The crystallinity of the naked AuNPs was characterized by using an Ultima IV X-ray diffractometer (Rigaku Co., Japan) with Cu-Kα radiation (λ = 0.15406 nm), after drop-casting NPs onto a glass slide.

    Article Title: Tailoring chickpea protein functionality for food applications through supercritical CO 2 processing.
    Article Snippet: The molecular weight of the proteins was estimated using SDS-PAGE polyacrylamide gels (4–20 % Mini PROTEAN® TGXTM Precast Protein Gels, Bio-Rad Laboratories) compared to standard proteins (Precision Plus ProteinTM KaleidoscopeTM Prestained Protein Standards (10–250 kDa)). .. Subsequently, the gels were stained in Coomassie blue solution (Coomassie G-250 stain for protein polyacrylamide gels, Bio-Rad Laboratories) for 30 min at room temperature on a shaker. .. After destaining in a solution of 50 % methanol and 10 % acetic acid, the resulting gels were imaged using a ChemiDoc Imaging System (Bio-Rad Laboratories), and the intensity and apparent molecular weights of the bands were analyzed using Image Lab software 6.1 (Bio-Rad Laboratories).

    Article Title: A dual fluorescent-Raman bioorthogonal probe for specific biosynthetic labeling of intracellular gangliosides.
    Article Snippet: Following SDS-PAGE gel separation, the gel was washed with water 10minutes for 3 times and scanned on a Molecular Imager FX (Bio-Rad) using a 405-nm laser for excitation. .. For Coomassie staining, the gel was incubated with Coomassie blue solution for 1 hours followed by destaining using the destaining solution (40% methanol, 10% glacial acetic acid) for overnight, followed by water for an additional 10minutes for 3 times prior to scanning.The gelwas scannedonaMolecular ImagerFX (Bio-Rad)using a 405-nm laser for excitation. siRNA treatment experiment HeLa cells were seeded in a 4-well chambered coverglass slide (10k cells/ well) or a 6-well cell culture plate (100k cells/well), and cultured in antibiotic-free complete growth media. .. For siRNA experiments: DMEM (Gibco, A14430 without phenol red) for HeLa, and DMEM/F-12 (Gibco, 11320033) for SH-SY5Y, respectively, were supplemented as above without antibiotics.

    Article Title: Peroxisomal H 2 O 2 -generating sulfite oxidase (SOX) from pepper fruits is negatively modulated by NO and H 2 S.
    Article Snippet: Nitric oxide and hydrogen sulfide are signal molecules that can exert regulatory functions in diverse plant processes including fruit ripening.. Sulfite oxidase (SOX) is a peroxisomal enzyme that catalyzes the oxidation of sulfite (SO3 ) to sulfate (SO4 ) with the concomitant generation of H2O2.. SOX requires the molybdenum cofactor (Moco) and it has been proposed that SOX functions as a mechanism of protection against sulfite toxicity.

    Article Title: Impact of Five Soy Proteins on Lean Chicken Breast Systems with Varying Moisture Contents: Cooking Loss, Texture, Microstructure, and T 2 NMR
    Article Snippet: 10 μL of each sample and 5 μL of Precision Plus ProteinTM All Blue molecular weight standard (Bio-Rad) were loaded onto the gel placed in running buffer (100 mL 10× Tris-Tricine-SDS running buffer, 900 mL deionized water) and electrophoresed at 120 V for approximately 60 min. .. The gel was stained with Coomassie Blue solution for 60 min, destained overnight, and then imaged using Gel DocTM EZ Imager (Bio-Rad), as described by Marciniak et al. [ ]. ..

    Membrane:

    Article Title: Hortaea werneckii.
    Article Snippet: The initiation of this study relies on a targeted genome-mining approach to highlight the presence of a putative vanadiumdependent haloperoxidase-encoding gene in the deep-sea hydrothermal vent fungus Hortaea werneckii UBOCC-A-208029.. To date, only three fungal vanadium-dependent haloperoxidases have been described, one from the terrestrial species Curvularia inaequalis, one from the fungal plant pathogen Botrytis cinerea, and one from a marine derived isolate identified as Alternaria didymospora.. In this study, we describe a new vanadium chloroperoxidase from the black yeast H. werneckii, successfully cloned and overexpressed in a bacterial host, which possesses higher affinity for bromide (Km = 26 μM) than chloride (Km = 237 mM).

    Western Blot:

    Article Title: Hortaea werneckii.
    Article Snippet: The initiation of this study relies on a targeted genome-mining approach to highlight the presence of a putative vanadiumdependent haloperoxidase-encoding gene in the deep-sea hydrothermal vent fungus Hortaea werneckii UBOCC-A-208029.. To date, only three fungal vanadium-dependent haloperoxidases have been described, one from the terrestrial species Curvularia inaequalis, one from the fungal plant pathogen Botrytis cinerea, and one from a marine derived isolate identified as Alternaria didymospora.. In this study, we describe a new vanadium chloroperoxidase from the black yeast H. werneckii, successfully cloned and overexpressed in a bacterial host, which possesses higher affinity for bromide (Km = 26 μM) than chloride (Km = 237 mM).

    Imaging:

    Article Title: Storage protein biosynthesis is affected by ionome composition in soybean ( Glycine max (L.) Merrill) seeds
    Article Snippet: .. After that, the gels were carefully removed from the cassette and stained with 0.25% Coomassie Blue solution (0.25% Coomassie Brilliant Blue R250, 90% methanol: H 2 O (1:1 v/v), 10% glacial acetic acid) for 30-min, then destained overnight (60% H 2 O, 30% methanol, 10% acetic acid), and imaged at a ChemiDoc MP Imaging System (Bio-Rad, USA). ..

    Electrophoresis:

    Article Title: Concanavalin A-functionalized nanogold – a glycan tag for surface enhanced Raman spectroscopy
    Article Snippet: Electrophoresis was carried out in Tris–HCl-Glycine-SDS running buffer (pH 8.3) at 150 V for 45 min (Mini-PROTEAN®, BioRad US), using a molecular marker (ColorBurstTM 8000–220,000 Da, Sigma-Aldrich). .. Following electrophoresis, the gel was stained using a Coomassie blue solution (Coomassie Brilliant Blue R-250, BioRad, US). .. The crystallinity of the naked AuNPs was characterized by using an Ultima IV X-ray diffractometer (Rigaku Co., Japan) with Cu-Kα radiation (λ = 0.15406 nm), after drop-casting NPs onto a glass slide.

    Incubation:

    Article Title: A dual fluorescent-Raman bioorthogonal probe for specific biosynthetic labeling of intracellular gangliosides.
    Article Snippet: Following SDS-PAGE gel separation, the gel was washed with water 10minutes for 3 times and scanned on a Molecular Imager FX (Bio-Rad) using a 405-nm laser for excitation. .. For Coomassie staining, the gel was incubated with Coomassie blue solution for 1 hours followed by destaining using the destaining solution (40% methanol, 10% glacial acetic acid) for overnight, followed by water for an additional 10minutes for 3 times prior to scanning.The gelwas scannedonaMolecular ImagerFX (Bio-Rad)using a 405-nm laser for excitation. siRNA treatment experiment HeLa cells were seeded in a 4-well chambered coverglass slide (10k cells/ well) or a 6-well cell culture plate (100k cells/well), and cultured in antibiotic-free complete growth media. .. For siRNA experiments: DMEM (Gibco, A14430 without phenol red) for HeLa, and DMEM/F-12 (Gibco, 11320033) for SH-SY5Y, respectively, were supplemented as above without antibiotics.

    Cell Culture:

    Article Title: A dual fluorescent-Raman bioorthogonal probe for specific biosynthetic labeling of intracellular gangliosides.
    Article Snippet: Following SDS-PAGE gel separation, the gel was washed with water 10minutes for 3 times and scanned on a Molecular Imager FX (Bio-Rad) using a 405-nm laser for excitation. .. For Coomassie staining, the gel was incubated with Coomassie blue solution for 1 hours followed by destaining using the destaining solution (40% methanol, 10% glacial acetic acid) for overnight, followed by water for an additional 10minutes for 3 times prior to scanning.The gelwas scannedonaMolecular ImagerFX (Bio-Rad)using a 405-nm laser for excitation. siRNA treatment experiment HeLa cells were seeded in a 4-well chambered coverglass slide (10k cells/ well) or a 6-well cell culture plate (100k cells/well), and cultured in antibiotic-free complete growth media. .. For siRNA experiments: DMEM (Gibco, A14430 without phenol red) for HeLa, and DMEM/F-12 (Gibco, 11320033) for SH-SY5Y, respectively, were supplemented as above without antibiotics.

    Recombinant:

    Article Title: Peroxisomal H 2 O 2 -generating sulfite oxidase (SOX) from pepper fruits is negatively modulated by NO and H 2 S.
    Article Snippet: Nitric oxide and hydrogen sulfide are signal molecules that can exert regulatory functions in diverse plant processes including fruit ripening.. Sulfite oxidase (SOX) is a peroxisomal enzyme that catalyzes the oxidation of sulfite (SO3 ) to sulfate (SO4 ) with the concomitant generation of H2O2.. SOX requires the molybdenum cofactor (Moco) and it has been proposed that SOX functions as a mechanism of protection against sulfite toxicity.

    Purification:

    Article Title: Peroxisomal H 2 O 2 -generating sulfite oxidase (SOX) from pepper fruits is negatively modulated by NO and H 2 S.
    Article Snippet: Nitric oxide and hydrogen sulfide are signal molecules that can exert regulatory functions in diverse plant processes including fruit ripening.. Sulfite oxidase (SOX) is a peroxisomal enzyme that catalyzes the oxidation of sulfite (SO3 ) to sulfate (SO4 ) with the concomitant generation of H2O2.. SOX requires the molybdenum cofactor (Moco) and it has been proposed that SOX functions as a mechanism of protection against sulfite toxicity.



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